FastQCFastQC Report
Wed 13 Oct 2021
SRR11130946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11130946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11545132
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA875790.7585794601568869No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG519750.4501897423087064No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT512780.4441525657740423No Hit
CCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAA470920.4078948599288428No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC462600.400688359388182No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT437730.3791468126999327No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC419110.3630188030764828No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC383840.3324691307124076No Hit
CACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGC373360.3233917117621522No Hit
CTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATG356540.30882280081336444No Hit
CTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAA349230.30249112786237525No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC328720.28472606463053No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG326170.28251734150809193No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA311820.27008786040731275No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA289860.2510668565764341No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG281820.24410288249627635No Hit
ATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAAC275600.2387153304093881No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA274110.23742474317313997No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG267320.23154347650594206No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT261070.2261299394411428No Hit
CCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACT247030.21396896977877775No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC241700.20935230536991695No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG240010.20788848494759524No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA238240.20635537125084408No Hit
GTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCG232360.20126231558028093No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC222910.19307704753830446No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC218460.18922260914816738No Hit
GGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGA217220.18814856339451122No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG214470.18576660708599954No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA214040.18539415573594134No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT210970.18273502632971195No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA209910.181816890443522No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC208470.18056961150379225No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG207750.17994597203392737No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA206030.17845616663369462No Hit
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC199980.17321586275496895No Hit
TTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAG197500.1710677712476566No Hit
TATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAAT196600.1702882219103255No Hit
AATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAA196290.17001971047191144No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA190840.1652991061514065No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG189270.1639392256407289No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG180280.15615239392672167Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC170990.14810571243360404No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG167000.1446497103714362No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG163830.14190396437217004No Hit
GTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAATAGCCA163820.14189530271286635No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC163050.14122835494648306No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG156300.13538173491649988No Hit
TTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGA153520.13297379363007716No Hit
ACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGT147460.1277248280920478No Hit
CATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTT147130.1274389933350264No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA143250.12407826952519903No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG142380.12332470516577897No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT142350.12329872018786792No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATG142060.12304753206806125No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG140420.12162701994225791No Hit
GAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCC135950.11775525823351349No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA135130.11704500217061183No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA133870.11595363309834829No Hit
AAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCT130530.11306063889091955No Hit
CGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAA126430.10950935857641124No Hit
ACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG121590.1053171154734307No Hit
ATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATT119360.10338556544871033No Hit
AATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAAT118770.10287452754979329No Hit
GCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCT118140.10232884301366152No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC116750.10112487237045016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)