FastQCFastQC Report
Wed 13 Oct 2021
SRR11130944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11130944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12154204
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG536740.4416085166910149No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT508010.41797060506800776No Hit
GGTGGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCTCACC478900.3940200444224895No Hit
CTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC477600.39295045566126746No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT404160.3325269182580776No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC388860.3199386812990797No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT370260.3046353344077489No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG341760.28118665771941953No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC334160.2749336772691984No Hit
CCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCCTCTTCCCCGCGGGGCCC318000.2616378662066228No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAG301570.2481199097859473No Hit
GGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACC299290.24624401565088097No Hit
GGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCT295370.24301879415550373No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGT288530.23739111175030467No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA275480.22665408610880647No Hit
GGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCTCACCCCG265870.21874735688161892No Hit
CCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCCTCTTCCCCGCGGGGCC257130.21155642936386454No Hit
CCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGG256700.21120264231207572No Hit
CGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCG256060.21067607553732023No Hit
GGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCAC242930.19987322904897764No Hit
CCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGC233150.19182663052224563No Hit
CCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTC231150.1901811093511348No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC230410.18957226651782377No Hit
GGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTC228720.18818180112823513No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT226100.18602616839407993No Hit
CCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCT222870.18336865170273595No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC216440.1780783011376146No Hit
CGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGG205240.16886338257939393No Hit
CTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCTCACCCCGAG204900.16858364398030506No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC202770.16683116393307204No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA201360.1656710715074389No Hit
CCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGC197360.16238002916521724No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCG196400.16159017900308403No Hit
GCCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCAC194770.16024907924862872No Hit
CTGGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCC190110.15641501491994045No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC184510.15180755564083012No Hit
GGCTCCGGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGG180760.14872220344499731No Hit
GTCGGGGGTCGGCGCGCGGCGCGGGCTCCGGGGCGGCGGGTCCAACCCCG177060.14567798927844225No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC176260.14501978080999792No Hit
CTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTC175460.14436157234155358No Hit
CCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG172060.14156418635066517No Hit
CCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTCT171370.14099648154663194No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA171220.1408730674587986No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC168670.1387750279656323No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG167280.13763139075171027Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGGTGG166390.13689913383056596No Hit
GGGACGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGC163510.13452958334416634No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG162880.13401124417526641No Hit
GGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGC150820.1240887515134681No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA148980.12257487203604613No Hit
CCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAA148880.12249259597749058No Hit
CCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTCTT145890.1200325418266799No Hit
CCCGTCCTTTCCGCCCGGGCCCGCCCTCCCCTCTTCCCCGCGGGGCCCCG144910.11922623645283557No Hit
CGGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCT143440.11801677839206912No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC142680.117391480347047No Hit
GGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCCTCTTCCCCG142550.11728452147092479No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC142100.11691427920742486No Hit
CGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGG141970.11680732033130264No Hit
CCCCTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTC141470.11639594003852495No Hit
GCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCTCACCCCGA141420.11635480200924717No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT141070.11606683580430278No Hit
GGCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTC140500.11559786227053619No Hit
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCCTCCTTCTTCCCG138830.11422385209265865No Hit
CCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAG137980.11352450559493654No Hit
CCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCG137590.11320362896656994No Hit
CCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCCTCTTCCCCGCGGGGCCCC135170.11121254834952582No Hit
GTGGCTCGGGGGGGGCGGCGCGTCTCAGGGCGCGCCGAACCACCTCACCC134420.11059547791035926No Hit
GCCGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCA134280.11048029142838149No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA132210.10877717701628178No Hit
GTCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC131130.10788859558388192No Hit
CACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAG129720.10672850315824878No Hit
CTCTTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTCTTC129420.10648167498258215No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG128420.10565891439702675No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCC123100.10128182808187192No Hit
GTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCG122030.10040147425532761No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA121610.10005591480939434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)