FastQCFastQC Report
Tue 14 Nov 2023
GSM4106518_pass.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4106518_pass.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639858
Sequences flagged as poor quality0
Sequence length35-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTGTC44250.16762265243054741No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTGTGATGTGTTTAGGCTAAGGCGTC43530.164895233001169No Hit
CTCTGATCTTCAGGAAGTGTACAACACTTGGTGCCCACTCTTCCTAGGTTTCTTGCAGCCTCCACGAGAGTTGGG41390.15678873636384988No Hit
CTCAGCTTTAAACTCTTTGGGGACATATGTTTCATCAACTGTCAGAGCAGAGAAGCATGGCCGCCTTTCCACC38470.14572753534470415No Hit
GTTCAGGATTGCAGACAGATAGTCTTCCACACAAGGCAGTCTCTGATCTTCAGGAAGTGTACAACACTTGGTGCC37170.1408030280416598No Hit
GTTGGGTTTTCCTTAAAGGAGGTGCACATGGCCTCAGCCTCTGGCCTTTCAAATGGTGGCAGGCTGGGGTTGTC31270.11845334105092017No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGT28910.10951346625462431No Hit
GTCATCTTTGTGTTGCAGGAAACATTCGTTTCTTTCGGGCTCTTGTTTTGTACAGCAGTCAGCCAGTTCACCAT28630.10845280314319937No Hit
GTCTTCAGTTGCTCCGCTGTAGCCTTGGGCTTGTGCTTCACCAGCTCAGCAAGAGCCGTTTGTTTCTTAATCTGC28200.10682392765065393No Hit
GTCCTTGTCAGCAGCCTTGCAACATGTATCCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGC27880.10561174123759687No Hit
CTCCACGAGAGTTGGGGTTGACACCTGAGGTGCTTTCTGGGTGTAGCGAACTAGAATGGCATTTTGGAATCCAT27820.10538445628514867No Hit
CCCAAATCATTATACCGATGGGCGATCTCACTCTTGTGTGCTTCTCGGCGAAACACACCCCTGGAAAAAGCAG26620.10083875723618466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)