FastQCFastQC Report
Mon 21 Oct 2024
G237_M02_CKDL240034113-1A_22VLHGLT3_L7_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG237_M02_CKDL240034113-1A_22VLHGLT3_L7_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33595050
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTGGGGG6082741.8106060267807311TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTAGGGG1891550.5630442580082482TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTGGGG915820.2726056368423324TruSeq Adapter, Index 5 (97% over 37bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT488190.14531605102537426No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC369360.10994476864895275No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351250.104554093534613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)