FastQCFastQC Report
Thu 3 Oct 2024
28_DW_073024_N30_S28_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename28_DW_073024_N30_S28_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34374421
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1681160.48907296503990566No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT962530.2800134437173502No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC772840.22482996877241945No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA766670.2230350294482051No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG608380.1769862538193734No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT590080.17166252778483163No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA580100.16875920615506512No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC539730.15701500834006774No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT479110.13937980220815938No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT476440.1386030618523No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT455980.1326509615972877No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC444980.12945090769674344No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA346580.1008249709864204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)