FastQCFastQC Report
Mon 30 Sep 2024
G232_M14_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG232_M14_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17319575
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTGGGGG2926351.68961998201457TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTGGGGT321410.1855761472207026TruSeq Adapter, Index 9 (97% over 37bp)
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT243800.1407655788320441No Hit
GGGGCATAGAAATAAGGATGTCTTCTGGCAACTTCATGCAAATAGTGTCC224890.12984729706127315No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG199520.11519913161841441No Hit
GGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTCCA196340.11336305885103994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)