FastQCFastQC Report
Mon 30 Sep 2024
G232_M13_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG232_M13_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18188940
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTGGGGG1681570.9245013728122694TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTAGGGG543330.29871449353288315TruSeq Adapter, Index 10 (97% over 37bp)
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT303010.1665902466004066No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG253080.1391394990582189No Hit
GGGGCATAGAAATAAGGATGTCTTCTGGCAACTTCATGCAAATAGTGTCC252360.13874365411068484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)