FastQCFastQC Report
Mon 30 Sep 2024
G232_M12_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG232_M12_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21638005
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCTGGGGG1517210.7011783202749051TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCTAGGGG1267040.5855623011456002TruSeq Adapter, Index 5 (97% over 38bp)
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT335620.15510672079057194No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG281690.13018298128686076No Hit
GGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTCCA265200.12256213084339336No Hit
GGGGCATAGAAATAAGGATGTCTTCTGGCAACTTCATGCAAATAGTGTCC264930.1224373503934397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)