FastQCFastQC Report
Mon 30 Sep 2024
G232_M11_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG232_M11_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16016015
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTGGGGG2332211.4561737111260198TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTAGGGG935200.5839155370421418TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCATCTCCATCTGGGGG356270.22244609536142415TruSeq Adapter, Index 3 (97% over 34bp)
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT248380.15508227233803165No Hit
GGGAAGACTGGGCCTGGCACATCTCACTTAGCCGCAGGAGCCGGTTTCTC186990.11675188865644794No Hit
CGGGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTC178320.1113385570630397No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG167940.10485754415190046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCGCTCATCTCCATCTGGGGG167070.1043143378674408TruSeq Adapter, Index 3 (97% over 34bp)
GGAGGATTGGCTTCAGCGCAGCACTTTTCCAGAGTGGCTTCATATTTCTT160930.10048067512424283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)