FastQCFastQC Report
Mon 30 Sep 2024
G232_M01_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG232_M01_CKDL240031680-1A_22T3CYLT3_L4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25362872
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTGGGGG1448950.5712878257635807TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTAGGGG564690.22264434406324332TruSeq Adapter, Index 4 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)