FastQCFastQC Report
Thu 30 May 2024
G224_M13_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M13_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6052697
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTGCTATCTCGTAT217560.359443071410976TruSeq Adapter, Index 11 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189550.31316618029945986No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT141560.23387921120122152No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC100960.1668016753523264No Hit
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG92430.15270878419983688No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTGCTATCGCGTAT87910.14524103882946726TruSeq Adapter, Index 11 (97% over 37bp)
GAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT85330.14097847620655718No Hit
GCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAAT81320.13435333042443723No Hit
GAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT80540.13306464870123186No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC69340.114560500880847No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC68760.11360225036871993No Hit
GCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGA64810.10707623394992348No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA63460.10484582327514495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)