FastQCFastQC Report
Thu 30 May 2024
G224_M10_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M10_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462523
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTGCTATCTCGTAT3235603.8234460337655802TruSeq Adapter, Index 11 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480340.5676085016253427No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTGCTATCGCGTAT458910.54228508448367TruSeq Adapter, Index 11 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184910.21850457599937984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTGCTATCTCGTTT101120.11949155116033362TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)