FastQCFastQC Report
Thu 30 May 2024
G224_M09_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M09_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6990973
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTCGTAT3043144.352956305223894TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTCGTTT868991.2430172452389674TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGCGTAT826911.1828253377605664TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTCGGAT400830.5733536662207106TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTCGGTT293340.4195982447650706TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGCGTTT259590.37132170300185685TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGCGGAT153350.21935430161152103TruSeq Adapter, Index 27 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120200.17193600947965326No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGCGGTT109160.15614421626288644TruSeq Adapter, Index 27 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTCGTA93080.13314312614281304TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)