FastQCFastQC Report
Thu 30 May 2024
G224_M08_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M08_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12711891
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTCGGTT666580.5243751696738117TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTCGGGT300170.2361332393425966TruSeq Adapter, Index 5 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285190.22434899732856425No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTCGTTT168430.13249798948087266TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCGCGGTT163290.12845453127312056TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)