FastQCFastQC Report
Thu 30 May 2024
G224_M06_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M06_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14889272
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTCGTAT1042670.7002827270534113TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT585960.3935450974365973No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCGCGTAT301030.20217912601771262TruSeq Adapter, Index 7 (97% over 36bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT291020.19545616468018048No Hit
GCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAAT184890.12417665551411781No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC175540.11789696635268669No Hit
GAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT166850.11206054936735657No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTCGTTT164160.11025387943749031TruSeq Adapter, Index 7 (97% over 36bp)
GAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT156870.10535773676510175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)