FastQCFastQC Report
Thu 30 May 2024
G224_M04_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M04_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825290
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTCGTAT563860.7205611549220541TruSeq Adapter, Index 10 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383110.48957929993648797No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT172490.2204263356374013No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT141890.18132235354855858No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC126320.1614253273680592No Hit
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAG125830.16079915249147314No Hit
GCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAAT125290.1601090822193171No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT111810.1428828835736439No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG106730.13639111138373144No Hit
GTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGA106590.13621220427613545No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG92180.11779755127286017No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC91710.11719693455450213No Hit
GAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT85230.10891609128862957No Hit
CTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTT85110.10876274233926156No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG82320.1051973792664553No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC80950.10344664542783719No Hit
TGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG80050.1022965283075771No Hit
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG78270.10002185222528495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)