FastQCFastQC Report
Thu 30 May 2024
G224_M03_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M03_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7831617
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428500.5471411587160098No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGTAT138230.1765025026121681TruSeq Adapter, Index 4 (97% over 39bp)
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG125520.16027341480054502No Hit
GTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGA125330.16003080845245624No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGT118630.15147574249353615No Hit
GTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG102170.13045837149595033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGTTT100830.1287473583041663TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGGTT99870.12752155780855984TruSeq Adapter, Index 4 (97% over 39bp)
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAG99040.12646175113006675No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT88060.11244165796156784No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC87540.11177768269311433No Hit
GTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACT80410.10267356026220384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)