FastQCFastQC Report
Thu 30 May 2024
G224_M02_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG224_M02_CKDL240018118-1A_HH7TVDSXC_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9194265
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTCGGTT609370.6627718474505575TruSeq Adapter, Index 2 (97% over 37bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT407080.44275426040036914No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTCGGGT264470.28764670150360033TruSeq Adapter, Index 2 (97% over 37bp)
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG221380.24078053003693062No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218410.2375502555125396No Hit
GCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAAT216600.23558163703134508No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGCGGTT209300.2276419050353672TruSeq Adapter, Index 2 (97% over 37bp)
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC194520.21156666682981187No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC177270.19280497135986402No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTCGTTT163630.17796963650710526TruSeq Adapter, Index 2 (97% over 37bp)
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC163540.17787174940030553No Hit
GAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT162000.17619679223951015No Hit
GTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAA152390.16574462450233923No Hit
GAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT148450.16145934449355115No Hit
GGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGA147170.160067172307955No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT141390.1537806447823725No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGA137880.1499630476171831No Hit
TGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATG133580.1452862191811961No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC129580.1409356811012082No Hit
CTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGA122140.13284368027243068No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGCGGGT119650.13013547031763822TruSeq Adapter, Index 2 (97% over 37bp)
GCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGA117770.1280907174200439No Hit
CCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGG112670.12254378136805932No Hit
GAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCA111040.12077093710046426No Hit
GGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGG108220.11770380775407278No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA105200.11441915150368191No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGAT103460.11252666743888717No Hit
GGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGT103220.1122656351540879No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA102520.11150429099009002No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG99750.1084915433696984No Hit
GGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACA94270.10253130620011495No Hit
AAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCAT92820.10095423614611934No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT92720.10084547269411964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)