FastQCFastQC Report
Thu 5 Sep 2024
G216M53_CKDL230039434-1A_22GGJKLT3_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG216M53_CKDL230039434-1A_22GGJKLT3_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9188484
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC126800.13799882548633705No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT119110.12962965381449212No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTGGGGG99260.10802652537676509TruSeq Adapter, Index 23 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)