FastQCFastQC Report
Thu 5 Sep 2024
G216M40_CKDL230039434-1A_22GGJKLT3_L2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG216M40_CKDL230039434-1A_22GGJKLT3_L2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14711512
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTGGGGG214550.14583817081480135TruSeq Adapter, Index 19 (97% over 37bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC190210.129293304454362No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT177080.12036832108079715No Hit
GGGCGATCTCACTCTTGTGTGCTTCTCGGCGAAACACACCCCTGGAAAAA177000.12031394189801836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)