FastQCFastQC Report
Sat 16 Sep 2023
HVY3LDSX5_19512525_S92_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3LDSX5_19512525_S92_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168530427
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT6435350.3818509283193117No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG5510970.3270014856130401No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT5031530.2985532102164555No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG4966310.29468328588522474No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC4895280.29046861668486723No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC4208370.2497098046277424No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG3020990.1792548712880197No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT2960250.17565077432575424No Hit
CGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGA2400910.14246151527284742No Hit
CAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCT2399800.142395651795269No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT2355060.13974093829359371No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT2275170.1350005480019344No Hit
GCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT2097670.12446832523601212No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA2069660.12280631081531646No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG1953740.11592802764334063No Hit
CGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA1748840.1037699857011577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)