FastQCFastQC Report
Sat 16 Sep 2023
225L32LT3_19538163_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename225L32LT3_19538163_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72043452
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500200.34704056102142355No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG2485520.34500290185983873No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT2344270.3253966786599843No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC2258370.3134733188520728No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG2152740.2988113340265816No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT2143090.2974718646185916No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT1574370.21853061677277763No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC1488050.2065489588144666No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT1193880.16571665666436972No Hit
CAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCT1133580.15734670792843186No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG1110650.1541639065268555No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1025700.14237241158294303No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG995020.13811387050137464No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA972580.13499908360859778No Hit
CGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGA846710.11752768315432749No Hit
GCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT844720.11725146096552952No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG808670.11224753638956668No Hit
GAGCTTTTAAAGCATGGGCAGTAGCTCGTCTGAGCCAGACATTCCCCAAT784950.10895507894319112No Hit
GTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGAT756700.10503383430322023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)