FastQCFastQC Report
Sat 16 Sep 2023
225L32LT3_19538149_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename225L32LT3_19538149_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57907131
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT2553250.44092151621188075No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG2407930.4158261613755308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2373430.4098683459209886No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC2338950.4039139842725069No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT2269430.3919085544058469No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG2224430.38413749076948744No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC1686420.2912283808362048No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT1604990.2771662094604549No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT1238520.21388039410897425No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG1191110.20569314684231202No Hit
CAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCT1156250.19967316287867898No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1067000.18426055333323282No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG1042510.180031367812023No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA966060.16682919414536354No Hit
GCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT929490.1605139097635488No Hit
CGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGA907960.15679588753930843No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG847520.1463584856241626No Hit
GTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGAT779390.13459309527871446No Hit
CGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA730150.126089824757507No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA645710.11150785556963616No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG641550.1107894639090305No Hit
CTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT614530.10612337192115423No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG611380.10557939746660909No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC610540.1054343376120637No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG591910.10221711726661091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)