FastQCFastQC Report
Wed 5 Jul 2023
G204M4_CKDL230019934-1A_COMBINED_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG204M4_CKDL230019934-1A_COMBINED_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16966338
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC281340.16582246563754652Illumina Single End PCR Primer 1 (96% over 33bp)
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC241820.14252928357315525No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT198280.11686670394047319No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG182490.10756004035755978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)