FastQCFastQC Report
Sun 30 Apr 2023
Waxman_l33_S18_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman_l33_S18_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49775770
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGACGCGGTGATCGTGTAG1883020.37830052654132723No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGGTGATCGTGTAG653050.13119837222005806No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)