FastQCFastQC Report
Sun 30 Apr 2023
Waxman_l28_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman_l28_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53761926
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACACAGTGTATGATCTC1778770.33086054245898855No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACACAGTGTATGATCTC873900.16254998007325855No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACACAGTGTATGATCGC625380.11632395759035864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)