FastQCFastQC Report
Sun 25 Sep 2022
D21_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD21_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26136921
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATATGCATATATATGCATATATATGCATATATATGCATATATATG1159350.44356793212176754No Hit
CATATATATGCATATATATGCATATATATGAGATCGGAAGAGCGTCGTGT1000370.38274209881110327No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT863730.33046356148836353No Hit
CATATATATGCATATATATGCATATATATGCATATATATGAGATCGGAAG458490.17541852003149108No Hit
ATATATATGCATATATATGCATATATATGCATATATATGCATATATATGC328930.12584879450796824No Hit
CTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGA327150.1251677655527979No Hit
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG269210.10299989046146636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)