FastQCFastQC Report
Thu 13 Oct 2022
G194M37_S57_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M37_S57_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38437677
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA702000.1826333053373647No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT646720.16825158294555626No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC527980.13736001788037294No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA509640.1325886577380834No Hit
CCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGGGT385200.10021417267229754No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)