FastQCFastQC Report
Thu 13 Oct 2022
G194M35_S55_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M35_S55_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39926072
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA878660.22007173658355372No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT876480.21952572744947213No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC661790.16575384625865525No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT588970.14751513747708517No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA528780.1324397751925108No Hit
CCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGGGT462100.11573890865096872No Hit
AGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTG402540.10082133799688585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)