FastQCFastQC Report
Thu 13 Oct 2022
G194M30_S50_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M30_S50_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43407459
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT713110.16428282521674442No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA663430.15283778762539404No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC586680.13515649464761345No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT474310.10926923872692018No Hit
CCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGGGT439530.10125679091236371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)