FastQCFastQC Report
Thu 13 Oct 2022
G194M29_S49_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M29_S49_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34821311
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTG883240.2536492666803958No Hit
GCTAGTTCTACGGGAAGGAACTTTAATGTAGAAAAGATTAATGGGGAATG576620.16559399501069905No Hit
GTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTG469890.13494322485445767No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG468760.13461871093825273No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT377510.10841349425356214No Hit
GACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGT373720.10732508032221992No Hit
GCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGGAGAATTCCTTA368300.1057685622462635No Hit
GCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACT359250.10316957911205583No Hit
CAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGT349690.10042413394487071No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)