FastQCFastQC Report
Wed 19 Oct 2022
G194M9_S39_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M9_S39_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37365525
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC15278464.088918863042871No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG9291952.486770893758351No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC8812042.3583343202055906No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC8064872.1583719217112565No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG7154591.91475698521565No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG4386131.1738440715070912No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT4155771.1121936597973665No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC3757381.005573988322123No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATG3583860.9591354597586946No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG3527490.9440493610085767No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC3502930.9374764572423376No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG3383010.9053827023707014No Hit
CACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT3290410.8806005000598814No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC3249910.8697616318785832No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT2973150.7956933563759643No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCA2906570.7778747923386597No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC2689790.7198587467993558No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG2445460.6544695946330207No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC2339520.6261172564817435No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG2206170.5904292793959137No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG2205830.5903382864284659No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT2192080.5866584237743214No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG2122780.5681119159974334No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC2100290.5620929988271274No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT2012560.5386141369618117No Hit
GTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT1947170.5211140483105751No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA1921580.5142654893782437No Hit
CTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACG1884670.5043873998826458No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT1882470.5037986218579827No Hit
CGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACA1588800.42520478435670317No Hit
GTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTC1483390.3969942881840948No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT1472750.3941467435557242No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA1442020.3859225850566799No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG1417600.3793871489829194No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA1397780.3740827942334545No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG1377550.3686687126703024No Hit
CATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGA1364360.3651387207860722No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA1312880.35136131500895545No Hit
CTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCA1241740.3323223747023493No Hit
CTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCT1239110.33161851733650205No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG1214970.3251580166476986No Hit
CACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCC1206980.32301968191267216No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTG1203300.3220348168532357No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG1192870.31924347376358286No Hit
CCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCT1149000.3075027046990508No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC1127330.3017032411561192No Hit
GACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCA1114760.298339177624294No Hit
CAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG1109180.2968458224526485No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT1094720.2929759450723628No Hit
GTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGG1084770.2903130626426365No Hit
CAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACC1060610.2838472094263362No Hit
GATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATG1053840.2820353788686229No Hit
AGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATC1022910.27375769509460923No Hit
GTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGC983450.2631971583431519No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG981360.2626378192197219No Hit
GGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGA977580.26162619152280076No Hit
GGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT975370.2610347372343892No Hit
GCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCT957960.25637536204830524No Hit
CAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTC943710.25256168620673736No Hit
GGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATG942780.2523127936781298No Hit
CCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCC923410.24712887079734597No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT923130.24705393541238882No Hit
CTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCA915170.24492362946860777No Hit
TGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGA912930.24432414638895075No Hit
GGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG882590.2362043621760968No Hit
TGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGA839860.22476868717889015No Hit
GACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGG832120.22269725903757542No Hit
CTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAG823250.22032341309268372No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG755270.20213017213059364No Hit
CTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATG753610.20168591234834785No Hit
CGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG753330.20161097696339073No Hit
AAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTT749750.20065287454143896No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG745530.19952349123958513No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA745200.1994351745358857No Hit
TGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA744210.1991702244247873No Hit
CTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTC740780.1982522659590625No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCA738830.19773039452811114No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG730680.19554923957310916No Hit
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA720580.1928462131871558No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC704720.1886016588820845No Hit
TGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGC696130.1863027483221499No Hit
CTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC690160.18470501886431412No Hit
CTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTAC686570.18374424017861382No Hit
CGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCC686560.18374156391486537No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG679430.1818333878622072No Hit
CTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGAC675750.18084852280277072No Hit
CAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCAT660730.1768287746525708No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG652160.17453521662013313No Hit
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATC644050.17236476672012505No Hit
GCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCAT624390.1671032321906356No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGA615750.16479094031195868No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGG603440.16149645963759376No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATG602210.1611672791965321No Hit
CCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCC602020.1611164301853112No Hit
AAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAAT596110.15953475830996622No Hit
CACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGA588770.1575703807185902No Hit
CTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTC587980.15735895588246118No Hit
CTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTG582580.15591377345828808No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACC578280.15476298004644656No Hit
GGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCA578110.1547174835627226No Hit
CTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGA568260.15208136377048095No Hit
GCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTA565010.15121157805222862No Hit
CAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCT563740.1508716925561731No Hit
AGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCT561200.15019192156406205No Hit
GCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGAC554520.14840417738008496No Hit
GGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGC553630.14816598990647126No Hit
GGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA550700.14738184462816994No Hit
GGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTA550000.14719450616577714No Hit
GCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG547790.14660305187736555No Hit
GGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAA547040.1464023320962304No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGC543820.1455405751692235No Hit
GACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCT541300.1448661567046094No Hit
GCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCA535120.14321222570805575No Hit
TGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCT533220.1427037355958467No Hit
ACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCAC531190.14216045405490757No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCAC530270.14191423779004844No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATA528810.14152350328277202No Hit
TGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCA528030.14131475471039148No Hit
GGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAA527200.14109262481926857No Hit
GACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACC524730.14043158767339678No Hit
CAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCC509280.1362967601820127No Hit
CTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTT507390.13579094633355213No Hit
CGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCC501370.134179835556974No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC500620.13397911577583882No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC498070.13329666851997932No Hit
TGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAA494170.13225292565807653No Hit
TGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTA493910.13218334280061633No Hit
TCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGG493780.13214855137188625No Hit
CAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCC491580.13155977334722316No Hit
TGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTC479460.12831614168407912No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG479040.1282037386066434No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTC478910.12816894717791333No Hit
CTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTG471220.12611090035534092No Hit
GGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGA466900.12495475441600246No Hit
TGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAG462760.12384678122413642No Hit
CAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTG462240.12370761550921604No Hit
GGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACT458290.12265049132857092No Hit
TGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATT457290.12238286495372405No Hit
AGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATA451800.12091359615581473No Hit
CTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCT450060.1204479262635812No Hit
CGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTG449450.12028467417492461No Hit
GCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGC445720.1192864277967458No Hit
GGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAG440800.11796970603249922No Hit
GTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAAC435450.11653790492706848No Hit
CACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGG432840.11583940008871815No Hit
CCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGC428510.1146805778856312No Hit
CATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGT413820.11074914643913072No Hit
CTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCG404780.10832980401051505No Hit
CCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGA395750.10591313784564782No Hit
GAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGA395730.10590778531815088No Hit
GAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGC392490.10504067586364703No Hit
CTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTG391070.1046606464113645No Hit
TGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCAC390530.10451612816894719No Hit
CCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAG381100.10199241145414122No Hit
AAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCT380460.10182113057423921No Hit
TGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGC379580.10158561936437398No Hit
GCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTC378940.10141433848447198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)