FastQCFastQC Report
Wed 19 Oct 2022
G194M5_S36_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M5_S36_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36582121
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC17291774.726836369055802No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC11233143.0706639453737523No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC10351472.829652769449863No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG7285541.9915575698850267No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG6679411.8258673410434567No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG4761981.3017233199791778No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC4093631.1190247826253705No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT3967291.0844887862024184No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG3763361.0287429752911266No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT3581830.9791203741303026No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG3316000.9064537291317799No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG3129180.8553850663825644No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCA3117180.8521047754448137No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC3086630.8437537014324566No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC3020630.8257121012748277No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG2829630.7735008038489621No Hit
CACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT2823440.7718087204402391No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATG2777600.7592780090580313No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC2734420.7474744288336918No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT2579300.7050712013117009No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC2507870.6855452695047398No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC2190450.5987761070496705No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT2166890.5923358025085532No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA2007330.5487188673395946No Hit
GTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT1977060.5404443334491185No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT1951480.5334518466001466No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG1926400.5265960385402476No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG1771090.484140873078409No Hit
CTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACG1696560.4637675327791956No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT1686130.460916413239134No Hit
GTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTC1606080.43903413910855527No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG1573640.4301664192735025No Hit
GGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGA1541920.42149551689471476No Hit
CTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCT1451780.396855064800644No Hit
AGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATC1442560.3943347079301389No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA1416000.38707433065458396No Hit
CGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACA1406960.3846031781481451No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA1397810.38210195630811017No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT1381700.37769816572417986No Hit
AGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCT1350090.3690573326789882No Hit
GGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATG1330250.36363391832857367No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA1304980.356726172328827No Hit
CATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGA1289790.35257387071679086No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG1276990.34907489371652345No Hit
GTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGG1260690.3446191651927454No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG1252540.34239130093085635No Hit
GGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT1219450.33334589867000874No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCA1190670.32547866757097No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG1119980.30615502037183684No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC1117740.30554269939679No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG1046130.28596756322576267No Hit
GGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG1038990.284015790117801No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG1031710.2820257469488989No Hit
GATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATG1020640.27899967855882385No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTG1015370.27755908412199504No Hit
GCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCT995110.2720208595887592No Hit
CAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTC978100.2673710471844976No Hit
GACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCA969920.26513498219526416No Hit
CACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCC963190.26329528569434235No Hit
CCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCT943200.257830867707206No Hit
GGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA939740.25688505048682114No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG883600.24153875604971073No Hit
CTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCA875170.23923435166594087No Hit
GTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGC869260.23761880837909863No Hit
TGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGA865020.23645977224776002No Hit
GGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAA860370.23518865950938164No Hit
GGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCA857430.23438498822963275No Hit
TGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGC846660.23144092711300146No Hit
CAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACC825590.2256812829414675No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG817790.22354909383192953No Hit
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA787620.21530189569926797No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT786300.2149410636961154No Hit
CAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG770960.21074775844735738No Hit
GGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGA764000.20884518970346197No Hit
CTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATG762650.20847615697296504No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG751450.20541455209773102No Hit
GCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCA742030.20283952371159672No Hit
GACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGG735160.20096155714973443No Hit
GGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGT735000.20091781993723107No Hit
GCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCAT709760.19401827466482877No Hit
CTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGA700020.19135577185368777No Hit
CTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAG694530.1898550387496668No Hit
GCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG689030.18835157206986441No Hit
GGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAG670970.18341473420854956No Hit
GGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTA659370.18024378630205723No Hit
GTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAAC647870.17710017415337945No Hit
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATC646400.176698338513505No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC640600.17511286456025882No Hit
TGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA631740.17269091641788623No Hit
GGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACT613510.1677076077682866No Hit
CCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCC610060.16676452412368326No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC604270.16518178374621853No Hit
TGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGA596990.16319174057731645No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGG593080.162122912446766No Hit
CGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG590290.16136024480373895No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCAC588740.16093654055761283No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATG580940.15880435144807487No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTC577690.15791593931910072No Hit
CTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTAC576940.1577109211354913No Hit
GGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCT573920.1568853812494907No Hit
CTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTC569090.15556506414704602No Hit
GGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGC566510.15485980159542964No Hit
AAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTT566480.15485160086808528No Hit
CTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCA562260.1536980318883096No Hit
GAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTT558190.15258546654525582No Hit
TGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTC547950.14978628494504187No Hit
GGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAA547030.14953479597314764No Hit
CAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCC544470.14883500057309415No Hit
GGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCAT544060.14872292396605435No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACC542180.14820901171914005No Hit
ACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCAC540070.14763222722925223No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG529530.14475103835559452No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA529180.14465536320324346No Hit
GTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTAC520630.14231815591009608No Hit
GTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTGATGCT513760.1404401893482338No Hit
TGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCA509410.13925108388329915No Hit
AAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAAT508980.13913354012469642No Hit
GCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGAC507280.13866883224184842No Hit
GACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCT497940.13611567246196576No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATA494960.13530106687909102No Hit
CAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCAT492390.13459853790325607No Hit
CTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGAC488470.13352697619692416No Hit
AAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCT484900.13255108964294332No Hit
CTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC482650.13193603509211507No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGA473560.12945121470676893No Hit
GAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGA467690.12784660572305254No Hit
CATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGT464430.1269554600182969No Hit
GCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACC460820.12596863916119025No Hit
CTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTC458650.12537545321661367No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGC454120.12413714338761275No Hit
GCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTA451990.12355489174616201No Hit
AGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATA451040.12329520204692342No Hit
GAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGC449320.12282502701251247No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC443200.12115207863425961No Hit
CGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCC440240.12034294020294777No Hit
GATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCA440230.12034020662716632No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG434630.11880940418954931No Hit
GGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAA428240.11706264926519705No Hit
CACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGA426350.11654600344250132No Hit
CACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGG423710.11582433943619617No Hit
GGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCAC417320.11407758451184391No Hit
TGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAG416560.11386983275245303No Hit
AGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCC413920.11314816874614787No Hit
GCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTT412740.11282560680393573No Hit
AAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGA400040.10935396556148289No Hit
AGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGG398680.10898219925520448No Hit
GACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAG395970.1082414002184291No Hit
CGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCC390720.10680627293316317No Hit
CTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTG385820.1054668208002483No Hit
GCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTC383870.1049337735228638No Hit
GAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCT382660.10460301085330728No Hit
GCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGC382420.10453740503455226No Hit
TGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAAT379330.10369273011808144No Hit
CAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCT378160.10337290175165074No Hit
CATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCA376520.10292459532349149No Hit
GTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATC376280.10285898950473649No Hit
CAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCA375230.10257196404768329No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGTCTGCTGTCTCTTGC373720.102159194104683No Hit
CAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCC371630.10158787676635807No Hit
CCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAG370040.10115323821710612No Hit
GACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACC369830.10109583312569548No Hit
CCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGC368840.10082520912333105No Hit
GAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAGT367480.10045344281705262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)