FastQCFastQC Report
Wed 19 Oct 2022
G194M2_S34_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M2_S34_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40078035
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC16996814.240928977680667No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG10664432.660916384747905No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC10380362.5900371612530404No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC9676882.414509593596592No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG9132602.2787045322955577No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG5044181.2585896489186656No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC4671371.1655686213158905No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT4644941.1589739866238453No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT4493731.1212450909831282No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG4223321.053774218222026No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC3775450.9420247275097194No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG3450880.8610402181643886No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCA3407910.8503186346336591No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG3307080.8251602155644607No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC3196900.7976688477865744No Hit
CACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT3107420.7753424038878154No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATG2947370.7354078112861571No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC2746870.6853804084955761No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG2454210.6123578663474893No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC2343690.5847816640711052No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT2315070.5776405954034423No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG2291130.5716672486562777No Hit
GTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT2215850.5528838926359538No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG2173360.5422820754560447No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT2147790.5359020221425527No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT1801390.44947063896720485No Hit
CTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACG1795130.44790868614192286No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA1729470.4315256474026234No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA1710620.4268223230006162No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT1698500.42379822264240247No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA1674580.4178298661598554No Hit
CTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCT1618350.4037997371877139No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG1527330.38108904291340634No Hit
CGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACA1468140.3663203547778727No Hit
CATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGA1439160.3590894613470945No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA1406380.3509104176389885No Hit
GTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTC1372570.3424743753030806No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTG1341900.3348218045121224No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG1300990.3246142182369969No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG1296930.32360119451964153No Hit
CAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTC1273680.3178000119017811No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGC1267750.3163203984426881No Hit
TGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGA1266240.3159436334640658No Hit
AGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATC1251670.3123082256902066No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC1239760.3093365231104768No Hit
GGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGA1207600.3013121776055138No Hit
GACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCA1169550.29181819917069285No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCA1107400.2763109518717672No Hit
GGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATG1089920.2719494605960597No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC1060930.2647160720329727No Hit
GTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGG1027720.2564297376355902No Hit
CCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCT1012620.2526620878493669No Hit
CAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACC1002260.25007713077749444No Hit
CTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCA1000240.2495731140511255No Hit
CAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG997330.24884703054927718No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG978110.24405138625184594No Hit
CACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCC960150.2395701286253181No Hit
GTTGTGTTGACTTGCAACCTCAGAAACAGACATCATGGTGCACCTGACTG952730.23771874045222027No Hit
CTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATG951310.23736443166437673No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGG931890.23251888472077034No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG930500.2321720613298531No Hit
GTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGC929140.23183272333586213No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG869280.21689686133564184No Hit
CTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAG853530.21296702794934932No Hit
GATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATG846620.2112428915239981No Hit
GCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCT840860.20980569531415402No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG840670.20975828780028763No Hit
TGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGC838110.2091195339292458No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT830420.20720077718381155No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG826050.20611040436488465No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT820580.20474556699199448No Hit
AGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCT801760.2000497279869135No Hit
CTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGA800470.19972785591908385No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG766270.19119450342313438No Hit
CCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCC763990.19062561325673777No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA740100.18466474217111695No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCAC734180.18318762384433268No Hit
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATC732140.18267861685334624No Hit
GGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA732110.18267113145641994No Hit
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA719550.17953724527662096No Hit
CTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCA717430.17900827722716445No Hit
CTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTC707520.17653560110918612No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC694560.17330190963703684No Hit
TGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGA685480.17103632950068534No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGG681510.17004576197410878No Hit
CAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCAT672490.16779515263160982No Hit
CGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCC666630.16633300509867813No Hit
CGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG660730.1648608770365114No Hit
CTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTAC656220.16373557236526193No Hit
GGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCA651360.16252293806320595No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG647710.16161221477050958No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATG638000.15918944129870638No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC636710.1588675692308767No Hit
GCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG633400.1580416804366781No Hit
GACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGG630160.15723325756864076No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG619280.154518553616713No Hit
CTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGAC613370.15304393042223752No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACC613010.15295410565912226No Hit
GCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCAT612640.15286178576369824No Hit
AAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTT608540.1518387815171078No Hit
CTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTG594620.14836555734331786No Hit
CAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCT591440.14757210526913309No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATA580740.1449023136987629No Hit
TGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTC572870.14293864457177105No Hit
CTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTG539510.13461488318975717No Hit
TGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCA539250.1345500097497295No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGA539030.13449511683893683No Hit
AAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAAT537770.13418073016803345No Hit
GGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGT534920.13346961746003766No Hit
CTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTT531220.1325464185057975No Hit
GCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCA525700.13116910547136357No Hit
CTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTC522700.1304205657787364No Hit
TGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCT517840.12920793147668042No Hit
GCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGAC513310.12807763654081344No Hit
GGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAG508710.12692987567878514No Hit
CACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGA508370.12684504118028742No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGC506410.126355995247771No Hit
GGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT505030.12601166698916252No Hit
TCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGG495320.12358889351735931No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTC493430.12311731351100422No Hit
GGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGA486620.12141812840874058No Hit
TGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATT477670.11918498499240295No Hit
TGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA475630.1186759780014165No Hit
CCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAG475190.11856619217983116No Hit
ACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCAC468780.11696681236991784No Hit
GGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGC468060.11678716284368733No Hit
TGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAA457430.11413483719947848No Hit
GACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACC456840.1139876243932618No Hit
GACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAG453440.11313927940828437No Hit
CGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC451980.11277499009120583No Hit
GGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG451240.1125903503003578No Hit
CTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGG450540.11241569103874478No Hit
AGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATA449970.11227346849714563No Hit
GCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTA446610.11143510404140324No Hit
GGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAA440740.10997046137616279No Hit
CGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCC440070.10980328751147604No Hit
GCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGC439000.10953630835443903No Hit
CTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCT436350.10887509829261839No Hit
GGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCT434710.10846589659398222No Hit
GTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTAC433820.10824382981850283No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC427850.10675423583017482No Hit
CAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCC426820.10649723720237282No Hit
TGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGC426680.10646230535005022No Hit
GAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGA423760.10573372671589312No Hit
GGAAAGGTGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT422820.10549918427886996No Hit
TGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTA420810.10499766268480977No Hit
GCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACC417490.10416927875830241No Hit
CTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTG416780.10399212436438064No Hit
CCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCC414000.10329847758254614No Hit
GCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGA408880.1020209698404625No Hit
GAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTT403600.10070353998143872No Hit
CAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTG402940.10053886124906074No Hit
GGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACT401820.10025940643047994No Hit
CTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCATGCCTCTC401040.10006478611039689No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)