FastQCFastQC Report
Wed 19 Oct 2022
G194M21_S45_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M21_S45_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37288193
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC16262084.36118746757184No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC10031352.690221540099838No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG8013632.1491065549891353No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC7894362.117120558778485No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG5581641.496892059102998No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT4571231.2259188853694252No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG4024221.0792209748538901No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG3481240.9336038354017315No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC3209970.8608542655848194No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT3121130.8370290295375804No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC3078260.8255320926921828No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG2927030.7849750187680051No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATG2782260.7461503967220937No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC2631840.7058105497362127No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC2629790.7052607778553388No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG2536380.680209952785859No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC2517260.6750823243164398No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCA2511000.6734035087192346No Hit
CACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT2419520.6488702737619921No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG2399170.6434127821640485No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT2374970.6369227921556831No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA2117320.5678258530790162No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT2064940.5537785110691741No Hit
GTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT1935070.5189497919622975No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT1885620.5056882214699973No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG1803130.48356593734644104No Hit
GTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTC1715270.4600035190764004No Hit
CTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCT1564510.4195724904127159No Hit
GGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGA1545120.41437245296386443No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT1438830.3858674513940646No Hit
CTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACG1387950.37222238149217907No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA1387740.3721660633970651No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG1367160.3666468900759015No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG1356810.3638712125310014No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGC1351560.36246326015315355No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC1340480.3594918101823813No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC1329050.3564264967197525No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGG1284780.34455410590692875No Hit
CGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACA1282430.3439238796044635No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG1273790.3416067922626339No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG1258520.33751166220363643No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA1246770.3343605306913103No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA1225700.3287099484815475No Hit
AGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATC1207810.32391218314065257No Hit
GTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGG1167680.3131500633457888No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG1163260.3119647015343436No Hit
AGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCT1128350.30260248867516853No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG1127380.30234235271202337No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT1103420.295916726240931No Hit
GGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATG1094650.29356477531641184No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTG1050630.28175942985491415No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCA1043900.2799545689972158No Hit
GATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATG1039080.27866193462364885No Hit
GCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCT1035810.27778498142830355No Hit
CAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTC1030730.27642261988935746No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG1007350.27015253863334165No Hit
GTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGC990000.2654995912513111No Hit
CATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGA980580.2629733224133441No Hit
GACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCA976050.26175846064731534No Hit
TGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGA934350.2505752960461238No Hit
GGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA921990.24726057387656195No Hit
CACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCC905310.2427873080360853No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG902180.2419479002374827No Hit
GGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGT890770.23888795040296No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT868660.2329584595316807No Hit
CTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATG868590.2329396868333094No Hit
GTTGTGTTGACTTGCAACCTCAGAAACAGACATCATGGTGCACCTGACTG843100.22610374281210138No Hit
TGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGC840800.2254869255799014No Hit
CCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCT828900.22229556685677956No Hit
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA813460.21815484595888035No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG810590.21738516532565683No Hit
GCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCAT804380.2157197587987168No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG797890.21397926147829152No Hit
GCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCA780230.2092431778606166No Hit
CTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCA777150.20841717913227922No Hit
GCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG757240.2030776873526695No Hit
CTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGA723040.1939058832912606No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC722770.1938334743118284No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATG717600.1924469764464049No Hit
GGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGA716760.19222170406594924No Hit
GGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAG713800.19142788710624836No Hit
CAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG712940.19119725109768662No Hit
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATC703280.18860661872244652No Hit
CAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACC703120.18856370969759784No Hit
GGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT701620.18816143758964132No Hit
GGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCA701600.18815607396153522No Hit
GGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG682140.1829372638143125No Hit
GACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGG670910.17992558663274458No Hit
GTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTAC664280.17814754391557672No Hit
GGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCT661100.17729472704670887No Hit
CCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCC651610.17474968551037054No Hit
GAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTT645570.17312986982233222No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTC644920.1729555519088844No Hit
GCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACC628810.16863514946943126No Hit
CAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCAT620350.1663663347805564No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATA615730.1651273366880503No Hit
TGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTC597700.16029202595041278No Hit
GGAAAGGTGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT595800.1597824812803345No Hit
GGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGC594580.1594552999658632No Hit
GCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGAC583920.15659648618531877No Hit
CTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTAC577490.15487207974921177No Hit
CTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCA574130.15397099022738914No Hit
CTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGAC572460.15352312728053086No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCAC559100.14994022370566468No Hit
CTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAG555180.14888895259687163No Hit
GCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTT550920.14774649981027504No Hit
GCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTA546830.14664963786258026No Hit
TGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGA540840.14504323124480717No Hit
CTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTC540180.1448662315173063No Hit
GGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACT537930.1442628233553715No Hit
GGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAA537000.14401341464843845No Hit
ACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCAC535860.14370768784639149No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGC528180.14164805465365404No Hit
GGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAA525730.14099101021065838No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC524700.1407147833631949No Hit
AAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTT514520.13798469665719656No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGG508300.13631660831620346No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG504900.13540479153816867No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC504860.1353940642819565No Hit
CGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG502790.13483892877297646No Hit
GATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCA502180.1346753381157408No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA490520.13154834292989206No Hit
GGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAA490310.13149202483477815No Hit
GCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGA488910.131116570867352No Hit
TGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCA485080.13008943608503637No Hit
GCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGC480030.12873511998824935No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGA478680.12837307509108847No Hit
GAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGC473760.12705362257699107No Hit
GTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATC472790.12679348661384585No Hit
GGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCAT471580.12646898711342758No Hit
GAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGA469570.12592994248876582No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG468400.1256161702445597No Hit
AAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAAT466820.12519244362417883No Hit
GACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAG464460.12455953550766057No Hit
TGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA464070.12445494475959186No Hit
GGTGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG456140.12232826621552834No Hit
CCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAG452180.1212662678505231No Hit
GTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAAC450620.1208479048582483No Hit
GCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTC440630.11816877261925779No Hit
GGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTA428140.1148191868670064No Hit
GTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAAC424560.11385909743601681No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACC421880.11314037126980116No Hit
AAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCT420160.11267909925267766No Hit
CTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTG412660.110667738712895No Hit
GACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACC411500.11035664828274194No Hit
GCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGA402600.10796983377553318No Hit
CACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGA401570.1076936069280697No Hit
CGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC397860.10669865391439053No Hit
GACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCA396720.10639292711234356No Hit
CACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGG390780.1047999295648357No Hit
TGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTA390190.10464170253570615No Hit
GGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTA388100.10408120339862005No Hit
CTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTC386750.10371915850145917No Hit
AGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGG382720.10263838743808261No Hit
GGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCAC380540.10205375197451913No Hit
AAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGA378510.10150934372175127No Hit
TGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA376480.10096493546898344No Hit
CTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTG374480.10042857265837474No Hit
GGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTA373120.10006384594716079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)