FastQCFastQC Report
Wed 19 Oct 2022
G194M1_S33_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M1_S33_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40359199
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC18216114.5134964150304375No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC12801863.1719806926792575No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG11147732.7621286537426077No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC10849022.6881157874317574No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG10159302.517220423527236No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG5404261.3390404502329196No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT4838321.1988146741960861No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT4710011.1670226656381362No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC4551501.12774785247844No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG4410811.0928883895837478No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC4237461.0499365956197495No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG4114931.0195767264855777No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC3490340.8648189474721737No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG3407920.844397333059063No Hit
CACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT3045240.7545343008417982No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC2746360.6804793127831897No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG2729250.6762398827588229No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATG2676450.6631573634551072No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCA2560860.6345170527294162No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG2545330.6306691071842134No Hit
GTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT2418960.5993577821006804No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC2268460.5620676465853547No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT2233250.5533434892996761No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT2143510.5311081619830959No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG2093500.518716934892588No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA2083740.516298651021295No Hit
CTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACG1974010.4891103017183269No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT1910380.4733443793074288No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA1833270.4542384500742941No Hit
CTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCT1708050.4232120662255958No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA1663860.41226288955833834No Hit
CATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGA1623490.40226021334070583No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG1618010.4009024064129717No Hit
CAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTC1615250.4002185474493683No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGC1512950.3748711662984194No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT1471190.36452408284911697No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC1451910.3597469811033663No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA1407420.34872347194006503No Hit
CGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACA1363750.33790313826595025No Hit
GTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTC1347480.33387183923050606No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG1343320.3328410952853648No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTG1304490.32321999254742395No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG1295250.3209305516692737No Hit
GGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGA1294820.32082400842494424No Hit
TGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGA1221230.30259024714539057No Hit
GACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCA1219360.30212690792005065No Hit
GGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATG1135540.2813584085254021No Hit
AGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATC1120450.277619483974397No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGG1103220.27335032095161255No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC1050380.2602578906484244No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCA1041710.2581096815127575No Hit
GTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGG1036480.25681381833172656No Hit
CAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACC1030450.2553197351612454No Hit
CACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCC1027220.2545194219538401No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG1018360.2523241355706787No Hit
GTTGTGTTGACTTGCAACCTCAGAAACAGACATCATGGTGCACCTGACTG998200.24732899183653273No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG994010.24629081464178712No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG991420.24564907742594197No Hit
CCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCT990540.2454310354375467No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT971520.24071835518836734No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG964450.23896658603160087No Hit
CTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATG961610.23826290506905254No Hit
CTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCA946890.2346156572631682No Hit
GATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATG942400.23350314757237872No Hit
CAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG939300.23273504511325907No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG897600.22240282816316548No Hit
CTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGA888650.22018524203119097No Hit
CTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAG876810.21725158618732746No Hit
GTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGC873000.21630756348757071No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG855840.21205574471386313No Hit
GCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCT833440.2065055850092565No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT825730.20459523986092987No Hit
TGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGC814720.20186723725612096No Hit
GGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA793830.19669121778160165No Hit
CAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCT773360.19161926380154373No Hit
AGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCT767550.19017969112816138No Hit
CTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCA733960.18185692932111958No Hit
CTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTAC727700.18030585790367148No Hit
GACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGG722740.17907689396908No Hit
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATC708160.17546433466134945No Hit
GGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCA695950.1724390020723652No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA688320.17054847892298358No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGG685190.16977294321425954No Hit
TGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGA682940.16921544949393075No Hit
CCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCC677240.1678031320690978No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG667960.16550378019147505No Hit
CGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG658450.16314744006688536No Hit
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA657240.16284763233284189No Hit
CAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCAT647030.16031784971748322No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCAC643320.15939860451640778No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATG632530.1567251124086977No Hit
GCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG626870.15532270598333728No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATA618320.1532042298460879No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC615560.15252037088248455No Hit
TGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTC609940.15112787545659667No Hit
CTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTC608490.1507686017257181No Hit
AAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTT602000.14916054206130305No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC601540.14904656556736917No Hit
GGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT597950.14815705336471124No Hit
GCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCA593890.14715108691825127No Hit
GCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCAT571870.1416950817086335No Hit
CTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTC571130.14151172821839203No Hit
CTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTG562750.1394353738289008No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG559960.1387440816156931No Hit
GGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG539740.1337340713823384No Hit
CTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTT530800.131518963000232No Hit
GCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGAC529910.1312984432619686No Hit
GACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAG525000.13008186807671776No Hit
CTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGAC523660.12974984959438862No Hit
GGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGT518820.12855061865821468No Hit
AAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAAT514340.12744058671729336No Hit
CGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC512210.12691282599538212No Hit
GGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAG507920.12584987130195524No Hit
GGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCT505180.12517096783808815No Hit
GGAAAGGTGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT500960.12412535739373817No Hit
GTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTAC499140.12367440691773887No Hit
CGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCC495210.12270065121956458No Hit
GGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGA492980.12214811299897206No Hit
GACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACC492520.12203413650503817No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGC483730.11985619437095368No Hit
CCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAG483480.1197942506242505No Hit
ACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCAC481700.11935321114772371No Hit
CACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGA478410.11853803144110964No Hit
TCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGG473150.11723473501047432No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACC472580.11709350326799103No Hit
CTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTG466140.11549783235291662No Hit
TGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCA464270.1150344931275767No Hit
GAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTT461680.11439275591173155No Hit
GGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGC460920.11420444692175381No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGA459230.11378570719404019No Hit
GCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTA458000.11348094396026046No Hit
GAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGA454890.11271036375127266No Hit
CCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGT451600.11189518404465858No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTC449490.11137237882248358No Hit
GGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACT445250.1103218128783973No Hit
TGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA440420.1091250596920915No Hit
CTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC429710.10647138958332647No Hit
GCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACC428760.1062360033458543No Hit
AGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATA427430.10590646261339329No Hit
CCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCC421800.10451148943763726No Hit
GGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCAT419690.10398868421546226No Hit
CTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTG417820.10352534499012234No Hit
CAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCC412180.10212789406449817No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC411560.10197427357267422No Hit
TGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATT407180.10088901913043419No Hit
GCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTT404800.10029931466181971No Hit
GGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAA404080.10012091667131451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)