FastQCFastQC Report
Wed 19 Oct 2022
G194M10_S40_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG194M10_S40_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31666378
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC12628753.9880626701291826No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG9475142.992176749737529No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC7763742.451729717873007No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC7239402.286147155825652No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG5206651.6442202515235558No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG4040801.276053737500386No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT3963671.251696673361254No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG3563131.1252092045386435No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC3417641.0792645751907592No Hit
CACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT3335121.0532053902722944No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCA3125270.986936365125181No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC2991490.944689664223676No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC2960840.935010628623204No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG2884640.9109472513717862No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATG2845830.8986913501758867No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT2470850.7802755338801299No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC2235840.7060611731471153No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG2082980.6577891541621843No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG2070690.653908066151424No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT1965500.6206898686044865No Hit
GTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGAT1890520.5970117580229731No Hit
CTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACG1885450.5954106907964024No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG1847680.5834832136469791No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT1846330.583056893971265No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT1845090.5826653114543129No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC1809810.5715241572623178No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA1705260.5385080668209039No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG1460100.4610884137112239No Hit
CGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACA1446310.4567336371718926No Hit
CTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCT1346570.42523650794543033No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG1341530.4236449144894311No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA1318180.4162711630613391No Hit
CATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGA1270190.40111628807058386No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG1230520.38858880545163704No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG1218680.3848498239994482No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA1156820.3653149090811712No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTG1107880.34986003135565424No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA1101110.34772211713003615No Hit
GTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTC1080110.3410904777300391No Hit
CTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCA1053410.33265882192147145No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT1039690.32832615084680666No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC1031860.3258534967276649No Hit
CACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCC1006640.3178892136006208No Hit
GACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCA999040.31548919172252665No Hit
CCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCT998320.31526182122881247No Hit
AGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATC991770.31319338132071817No Hit
CAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACC941100.2971921828255824No Hit
CAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG930970.29399320629596476No Hit
CAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTC908970.2870457745435869No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC907080.28644892699758717No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGC899300.28399206249606446No Hit
CTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAG874090.2760309372925442No Hit
CTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCA855950.2703024640203562No Hit
GGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATG853780.2696171946156899No Hit
GGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGA841270.2656666322874059No Hit
GTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGG838710.264858203865311No Hit
GTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGC834920.26366135084978776No Hit
TGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGA805850.2544812671660775No Hit
CTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATG804090.2539254726258873No Hit
GATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATG797810.2519422966529358No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT796460.2515159769772217No Hit
CCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCC790860.24974753980388917No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA771380.24359590477951096No Hit
GCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCT767120.2422506293583687No Hit
TGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGA758260.23945270911627467No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG738560.2332315997743727No Hit
CGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG729240.23028841505018352No Hit
CTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTAC716530.22627469425142338No Hit
CTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTC697520.22027148163266413No Hit
CGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCC686780.2168798717680942No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC666910.21060507772628748No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGG653790.20646188206305122No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCA652960.20619977441057516No Hit
CTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGA652480.20604819408143235No Hit
TGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGC646260.20408396564962372No Hit
AAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTT637160.20121025524295835No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG630940.19924602681114967No Hit
CTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTG628540.1984881251654357No Hit
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCA620690.19600915519924636No Hit
GACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGG619770.19571862623505598No Hit
CTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGAC616180.19458493169000887No Hit
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATC615040.19422492840829475No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT613360.19369439725629498No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG609500.19247543877610507No Hit
CAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCAT591870.18690801960363135No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGG585840.18500379171877504No Hit
CTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTC572430.18076901627334835No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG566620.17893426270601584No Hit
AGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCT561290.17725108946782608No Hit
TGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCA556680.17579528672335057No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG547700.17295947139897086No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACC545070.17212893751220934No Hit
TGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATT542830.1714215626428763No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGA538750.1701331298451626No Hit
TCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGG537430.16971628394001992No Hit
GCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG536610.16945733421106765No Hit
GCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCAT526550.16628046314611666No Hit
GCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCA524740.1657088789883074No Hit
GTTGTGTTGACTTGCAACCTCAGAAACAGACATCATGGTGCACCTGACTG523030.16516887406573622No Hit
TGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCT515320.16273411502888016No Hit
GGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCA514760.1625572713115469No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG509300.16083304506754767No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATA496080.15665827016907333No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATG495960.15662037508678764No Hit
CACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGA492270.15545510130650242No Hit
TGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAA490110.15477298982535986No Hit
CCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCC477690.15085084880879018No Hit
AAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAAT477460.1507782165677426No Hit
TGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTA475940.15029821219212378No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG475460.15014663186298097No Hit
GCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTA473150.1494171515289813No Hit
GGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA467110.1475097657206012No Hit
CTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTT465190.14690344440403003No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCAC458080.14465816077860247No Hit
ACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCAC457070.14433921050269782No Hit
TGAACGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTC456580.14418447225003125No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG456020.144007628532698No Hit
CAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCT454350.14348025530422204No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCTGCTC448030.1414844476371753No Hit
CTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCCTGTCT446190.14090338970879462No Hit
GCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGAC445890.14080865200308035No Hit
CGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTG439800.13888547657708122No Hit
GGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGC436070.1377075711027008No Hit
CGCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC430680.1360054503233682No Hit
GGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAG426760.13476754430203544No Hit
CTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTG420160.13268331477632206No Hit
GCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGC415680.13126856503765602No Hit
CGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGCTGCCC414880.13101593115575136No Hit
CAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCC412550.1302801349747041No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC410930.12976855136384716No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC408720.12907065026508557No Hit
CAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTG395870.12501271853699214No Hit
GGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACT392580.12397376169765928No Hit
AGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATA392030.12380007590384982No Hit
CCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAG391780.12372112781575463No Hit
CTGCCCGCTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCG389580.12302638464051682No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGC388060.122546380264898No Hit
GGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAA380250.12008004199280384No Hit
CACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGG377070.11907582231223286No Hit
TGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA373340.11789791683785243No Hit
TGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGC373220.11786002175556674No Hit
TGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCAC371370.11727580590366225No Hit
GGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGT369970.1168336966103291No Hit
CTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTG362200.11437999003233018No Hit
GGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACCTCGATGA360960.11398840751537798No Hit
CAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCA356950.11272208018233093No Hit
GGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT354390.11191365176023604No Hit
CTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC346470.10941257632938002No Hit
CTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGC343610.10850941020157089No Hit
GAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGC339690.10727150418023812No Hit
AGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGG338980.10704729161004772No Hit
TGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGAC335380.1059104391414768No Hit
GCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACC333450.10530095990138183No Hit
GAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGA331170.10458095333795359No Hit
CTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCATGCCTCTC326050.10296409649376383No Hit
TGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTA324990.10262935660024015No Hit
TGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACA323880.10227882708909747No Hit
GGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTG321700.1015903997609073No Hit
TGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACT321240.10144513527881212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)