FastQCFastQC Report
Wed 27 Apr 2022
G192M37_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG192M37_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17632450
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATCGTGGTAACCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA832390.47207846895922007Illumina Single End PCR Primer 1 (100% over 34bp)
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC245870.1394417678768407No Hit
CAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATT200170.1135236453243877No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197350.11192432135069148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)