FastQCFastQC Report
Thu 2 Jun 2022
RX20220513-M3_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRX20220513-M3_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64913955
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC6164170.9495908853496909No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC5513680.8493828484183409No Hit
TCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG4984770.7679042202866857No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA3802440.5857661884875139No Hit
ATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA3047850.46952153816540676No Hit
TTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG2988520.4603817468832395No Hit
TTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA2950370.4545047363082406No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG2730490.4206322045852852No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG2612870.4025128341047776No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA2007420.30924321280378003No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT1976180.3044306882857469No Hit
TTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGAC1891540.29139188946352135No Hit
TCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT1833220.28240768876276295No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1386130.21353343822603324No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1312670.2022169193049476No Hit
TCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCTT1272150.19597481003891998No Hit
TGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1190520.18339970195930905No Hit
CCTCATTTCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTA1181230.18196857671050856No Hit
TTTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC1138480.17538293576473657No Hit
TCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCC1099710.16941041413976393No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT1081210.16656048764861114No Hit
GTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA1045410.16104549476302898No Hit
TTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGG1010160.1556152294217784No Hit
TTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCTTCTTA996770.15355249884250619No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC980050.1509767814948265No Hit
TCGGCTTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG912900.1406323185823449No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA886450.13655769395039943No Hit
TTGACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGC852900.13138931374617369No Hit
TTTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA844380.1300768070594374No Hit
TTCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCT805900.12414895995783957No Hit
TCTTTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG779720.12011592884765686No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGCCCGCACGCGCGG772390.11898674175683796No Hit
TTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG761640.11733070339035728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG760500.11715508629847003No Hit
TCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG752580.11593500966009541No Hit
TTTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG750970.11568698903032483No Hit
GCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGAAA748820.1153557813570287No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA734080.11308508316894264No Hit
TGGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG714290.11003643207381218No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC710300.10942177225220677No Hit
CTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA708990.10921996664661705No Hit
TATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA674810.10395453489161152No Hit
TCGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC671690.10347389864013062No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC667450.10282072629837452No Hit
ATTTGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC661260.10186715629944285No Hit
TCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG657020.10121398395768674No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)