FastQCFastQC Report
Thu 2 Jun 2022
RX20220513-M2_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRX20220513-M2_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77498851
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC14335571.8497783921983566No Hit
TTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC11744711.5154689196617896No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA9982321.288060386856574No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG6591850.8505739007666062No Hit
TCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG5646110.7285411237903385No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT4669370.6025082875099658No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG3861430.4982564193113005No Hit
ATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA3328740.4295212067079549No Hit
TTTCTCTTTTGTCAGAGGCCAGGATAATAGTATGCCATTCCCCATTAATC3041080.3924032370492822No Hit
TTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG3036020.39175032414351535No Hit
TTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA3031850.3912122516500277No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA2867930.37006097032328905No Hit
TCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCC1932390.24934434189224303No Hit
TTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGAC1927290.2486862676196322No Hit
TCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT1848760.23855321416313643No Hit
TTTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC1842150.23770029829216435No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT1725500.22264846223333043No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1721230.22209748632273268No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1670670.2155735186319085No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA1654020.2134250996830908No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC1586290.2046856152744768No Hit
TTCGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC1362640.17582712290792543No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC1309180.16892895612091074No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT1303320.1681728158782638No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT1249230.161193357563456No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG1221300.15758943316462848No Hit
TTTCTCTTTTGTCAGTGGCCAGCATAATAGTATACCATTCCCCATTAATC1190560.15362292274500947No Hit
TGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1188560.15336485440280914No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1148790.1482331654181557No Hit
TGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA1097720.1416433903000704No Hit
TCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCTT1079320.1392691615518274No Hit
CCTCATTTCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTA1075340.13875560555084873No Hit
TTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCATGGACA1049590.13543297564501958No Hit
TCGGCTTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG1043760.13468070642750563No Hit
GCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG1036570.13375295073729546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1036030.13368327228490134No Hit
TTGACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGC1033150.1333116538721329No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG1031820.13314003842456967No Hit
GTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA1001480.12922514167339075No Hit
GTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCTTCCTGGACC996830.128625132777775No Hit
GCGTACGTCGGGAAAAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGA995960.12851287304891784No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC991900.1279889943142512No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA974440.12573605768684237No Hit
CGTGCTGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA927550.11968564540395574No Hit
CTCTTTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC908390.1172133506856766No Hit
GCGTACGTCGGGGAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGAAA858940.11083261092477359No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC842680.10873451530268494No Hit
CCGCCGCTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG837040.10800676257768002No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT829820.10707513586233684No Hit
TCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGAC827020.1067138401832564No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGCCCGCACGCGCGG820460.10586737602083932No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)