FastQCFastQC Report
Thu 2 Jun 2022
RX20220513-M1_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRX20220513-M1_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93860120
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC8607180.9170220536688No Hit
TTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC7782430.829151933749925No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA6055350.6451462026683963No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG4215990.44917798954444127No Hit
ATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA3018360.32158066706072824No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG2977760.31725508128478846No Hit
TTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA2905620.30956917591837724No Hit
TTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGG2904950.3094977930989221No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT2782350.2964358025538429No Hit
TTTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA2532450.26981107631228257No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA2492480.26555261169493494No Hit
TCTTTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG2290450.24402802809116372No Hit
TTTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG2274580.24233721414377052No Hit
TTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG2257420.24050896163354574No Hit
TCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG2138500.22783904388786205No Hit
TATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA1978090.210748718412037No Hit
TTTCTCTTTTGTCAGAGGCCAGGATAATAGTATGCCATTCCCCATTAATC1946920.20742781918454822No Hit
ATTTGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC1858660.1980244644903501No Hit
CTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGG1787370.19042911941727753No Hit
TCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG1786300.19031511998919243No Hit
TTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGAC1775420.18915594823445783No Hit
TCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1759380.1874470222283969No Hit
TCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT1742880.1856890871224115No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC1735720.18492624982793543No Hit
ATTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA1713350.1825429159903056No Hit
TCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCTT1657860.17663092695811597No Hit
CCTCATTTCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTA1629000.1735561386454652No Hit
CTATTTGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGG1586460.16902386231767016No Hit
CCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA1456850.1552150157063511No Hit
TCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCC1435220.15291052259468665No Hit
TTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC1432210.1525898326147463No Hit
CTTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA1423350.15164587473359292No Hit
TTTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC1389800.148071406684756No Hit
TTTGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACG1387280.14780292205038734No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC1376710.14667677816734093No Hit
CTTTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGG1369410.14589902505984437No Hit
CTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1352850.1441346974625645No Hit
TTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTT1340320.14279973219723135No Hit
TTTTGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC1335100.14224358545461055No Hit
TTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCTTCTTA1297770.1382663904542206No Hit
TTGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC1263200.1345832500533773No Hit
ATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC1231830.1312410425215736No Hit
GACCGGTCCTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGC1225780.13059646631604563No Hit
TTCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1214090.1293509959288354No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1212070.12913578205525414No Hit
TCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA1180550.1257775932952142No Hit
ATTCTGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC1171130.12477397216197891No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA1150910.12261970259573503No Hit
TTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACG1142050.12167574471458166No Hit
CTGTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGG1121050.11943837276150937No Hit
CTTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA1116600.1189642629905012No Hit
TCTGTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGG1114860.1187788807429609No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1108100.1180586600571148No Hit
TTCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTTAAGATCT1084810.11557730801963603No Hit
TCTCTGTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA1081430.11521719767671297No Hit
TGACCGGTCCTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCG1078830.1149401897206183No Hit
TTCGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC1056200.11252915508737896No Hit
CTCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1043320.11115690028949463No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT1024170.10911663015133582No Hit
GCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1011710.10778912279251293No Hit
CATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA997890.10631671896434822No Hit
GTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA973760.10374587204874659No Hit
TCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTACTCTATTAGCT971880.10354557398818583No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT968670.10320357570393049No Hit
TTTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA965340.10284879243708615No Hit
AATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGA958470.1021168521838668No Hit
GTTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC955080.10175567642572798No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC944110.10058691593405164No Hit
ATTCAGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGAC942560.1004217765756106No Hit
TCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGC941750.10033547794313495No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG940020.10015116111081043No Hit
GGTCCTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCT939800.10012772197606397No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)