FastQCFastQC Report
Tue 16 Mar 2021
G184_M19_CKDL200149567-1a-25_H7T5YBBXX_L3_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG184_M19_CKDL200149567-1a-25_H7T5YBBXX_L3_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29595255
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG560340.1893344051267678Illumina Single End PCR Primer 1 (100% over 50bp)
GGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGGAGAATTCCTT302370.10216840503655061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)