FastQCFastQC Report
Thu 10 Nov 2022
G164-M4-i7-02-i5-04_TCCGGAGA-GGCTCTGA_H75HLDSXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG164-M4-i7-02-i5-04_TCCGGAGA-GGCTCTGA_H75HLDSXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25458340
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG871380.3422768334463284No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT639670.2512614726647535No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA359250.14111289267092827No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT345180.1355862165404343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)