FastQCFastQC Report
Thu 10 Nov 2022
G164-M4-i7-02-i5-04_TCCGGAGA-GGCTCTGA_H75HLDSXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG164-M4-i7-02-i5-04_TCCGGAGA-GGCTCTGA_H75HLDSXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25458340
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT354950.13942385874334304No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326430.1282212430189871No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC313510.12314628526447521No Hit
GAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT299740.11773744871032439No Hit
AAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCAT292540.11490929887808868No Hit
CAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG261550.10273647064184074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)