FastQCFastQC Report
Thu 10 Nov 2022
G142-M13-Index-27_ATTCCT_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M13-Index-27_ATTCCT_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16298935
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC334910.2054796831817539No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA293660.18017128112971798No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG235600.1445493217808403No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC196630.12063978413313509No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT196420.12051094135905199No Hit
CTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATAG171410.10516638050277519No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG169750.10414790905049931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)