FastQCFastQC Report
Tue 15 Nov 2022
G137-M14-Index-19_GTGAAA_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M14-Index-19_GTGAAA_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13583523
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG280830.20674312547635837No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC194680.14332069817233717No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT175270.1290313271453952No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC166200.1223541197670148No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT162340.11951244165449566No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG147860.10885246780235143No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT147490.10858007896773171No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG147330.10846228920140968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)