FastQCFastQC Report
Mon 7 Feb 2022
665_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename665_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31885811
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1081400.33914771683241807No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG957490.3002871716200037No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG661600.2074904100761307No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC600250.18824987703778337No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA593820.18623330609342192No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAA570680.17897615964668423No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC536850.1683664248025556No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACC520320.1631823007418566No Hit
TCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAA498330.1562858162836128No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCA433120.1358347134404077No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA426400.13372719295112176No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC420670.13193015539106093No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC401570.12594003019085825No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA324550.10178508553538124No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)