FastQCFastQC Report
Mon 7 Feb 2022
662_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename662_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45110188
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT1381320.30621020688275563No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG1015570.2251309615468683No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG788800.17486072104155273No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC723900.16047372713232763No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC695350.15414477988874709No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA692980.1536193996797353No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAA672580.1490971396527986No Hit
TCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAA629050.13944743480120278No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC589000.1305691743071432No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCA570230.1264082517235353No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA542790.12032536862847923No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACC519370.11513363677402542No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC503980.11172199060664521No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)