FastQCFastQC Report
Mon 7 Feb 2022
661_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename661_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30328396
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT731660.24124586080978366No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC516480.1702958507927686No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACC479610.15813892696468354No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG471340.15541210949632814No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAA411260.13560229166092397No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA400630.1320973255558916No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC396490.13073226820172093No Hit
TCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAA359260.11845664373414276No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCA326070.10751310422087604No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC320430.10565346086881745No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC316980.10451591307367525No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA311200.10261010836181379No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)