FastQCFastQC Report
Mon 7 Feb 2022
661_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename661_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30328396
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT651170.21470637616311788No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC495420.16335186338242222No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG418210.137893873451138No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACC410460.13533851246205042No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAA386760.12752405369542127No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA361820.11930073717053814No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCA315520.104034516035731No Hit
TCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAA309570.10207265824410892No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT305800.10082959876941727No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)